PinnyPeptide

PE22-28 vs Semax

Side-by-side comparison: effects, dosing ranges, side effects, regulatory status, and reconstitution.

Peptide A

PE22-28

Cognitive

Fast-acting antidepressant peptide acting via TREK-1 channel inhibition.

Peptide B

Semax

Cognitive

Neuroprotective ACTH analog with nootropic and neurotrophic properties.

Typical vial

5 mg

Typical dose

50-200 mcg

Half-life

Hours; precise value species-dependent

FDA status

Not FDA approved.

Typical vial

5 mg

Typical dose

200-600 mcg

Half-life

~2-3 minutes (very rapid degradation; biological effects last hours due to downstream gene expression changes)

FDA status

Not FDA approved for human use. Approved in Russia for strok…

PE22-28 effects

  • Selective TREK-1 potassium channel inhibition
  • Fast-onset antidepressant-like effects in rodent models
  • Increased serotonergic neuron firing in dorsal raphe
  • Enhanced BDNF / TrkB signaling downstream
  • Anti-anxiety effects in some assays

Semax effects

  • Upregulates BDNF and NGF expression for neuroprotection
  • Enhances memory formation, learning, and attention
  • Promotes neuronal survival after ischemic injury
  • Modulates serotonergic and dopaminergic neurotransmission
  • Supports optic nerve health and visual function

PE22-28 side effects

  • Essentially uncharacterized in humans
  • Theoretical: TREK-1 inhibition off-target in heart, immune cells
  • Subjective reports of mood activation, occasional anxiety
  • Injection-site reactions
  • Sleep disturbance with late-day dosing

Semax side effects

  • Mild nasal irritation with intranasal administration
  • Occasional headache
  • Rare anecdotal reports of hair thinning at high doses
  • Mild dizziness in sensitive individuals

PE22-28 dosing ranges

Antidepressant research (preclinical)

100-500 mcg/kg · Daily SubQ or IP · Per protocol

Self-experimentation (no clinical guidance)

50-200 mcg · Once or twice daily · 2-4 weeks per cycle

Semax dosing ranges

Cognitive enhancement

200-600 mcg · Once or twice daily (intranasal) · 10-20 days per course

Neuroprotection / stroke recovery

600-1200 mcg · Two to three times daily (intranasal) · 10-14 days

Full PE22-28 guide → Full Semax guide →

PE22-28 vs Semax — common questions

What is the difference between PE22-28 and Semax?

PE22-28: Fast-acting antidepressant peptide acting via TREK-1 channel inhibition. Typical dose 50-200 mcg. Semax: Neuroprotective ACTH analog with nootropic and neurotrophic properties. Typical dose 200-600 mcg. Both fall under the Cognitive category.

Can you stack PE22-28 and Semax?

Stacking PE22-28 with Semax is a protocol-design question best raised with a clinician — it depends on your goal, current bloodwork, and whether both peptides target overlapping mechanisms. Both peptides should be tracked independently with separate injection sites and timing. PinnyPeptide supports multi-peptide stacks with automatic injection site rotation.

Which is dosed more frequently, PE22-28 or Semax?

PE22-28 is typically dosed: Daily SubQ or IP for Antidepressant research (preclinical); Once or twice daily for Self-experimentation (no clinical guidance). Semax is typically dosed: Once or twice daily (intranasal) for Cognitive enhancement; Two to three times daily (intranasal) for Neuroprotection / stroke recovery.

Are PE22-28 and Semax FDA approved?

PE22-28: Not FDA approved. Semax: Not FDA approved for human use. Approved in Russia for stroke recovery, cognitive impairment, and optic nerve disease.

Related peptide comparisons

adamax vs pe22-28colivelin vs pe22-28dihexa vs pe22-28dnsp-11 vs pe22-28p21 vs pe22-28pe22-28 vs pinealon

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